|Every living thing is
connected via webs of genetic and epigenetic relationships.
function as cross-kingdom master regulators of the fellowship of the
Produced by bacteria, fungi, plants, and animals, microRNAs
are capable of surviving digestive and assimilative processes intact, entering
the tissues of organisms, affecting the expression of a wide range of genes.
"The viral network we have detailed is a snapshot
of the early stages of an epidemic,
before the evolutionary paths of
COVID-19 become obscured by vast numbers of mutations.
It's like catching
an incipient supernova in the act." - Peter Forster
|MicroRNAs are short
non-coding RNA molecules involved in the posttranscriptional epigenetic
regulation of gene expression.
Recent data show that microRNAs can be
found in body fluids, and these microRNAs might enter cells giving rise to
a hormone like way of
MicroRNAs released in body fluids might affect other
individuals, and there are some data of potential cross-species action of
MicroRNAs wander via the food-chain.
microRNAs may influence gene expression.
MicroRNA genes, located in the
non-protein coding "dark matter" of the genome, may facilitate interindividual
and cross-species epigenetic communication via information transfer by coded
HIV-infected cells persist only for a day or
HTLV is thought to replicate by promoting proliferation of infected
"Assume about 10 years between HIV infection and diagnosis of AIDS."
The simian foamy virus (SFV), a spumavirus, has been co-speciated
with Old World primates for about 30 million years, making them the oldest
known vertebrate RNA (ribonucleic
acid) or retroviruses.
late 1950's SV40, a
retrovirus, is identified in the injected form of the
This is believed to be
due to kidney cells from infected monkeys
being used to amplify the vaccine virus
Both the Sabin vaccine (oral, live virus) and
the Salk vaccine (injectable, killed
virus) are affected; the technique used to inactivate
the polio virus in the Salk vaccine, by means of
not reliably kill SV40.
When two or more vaccines are mixed together in
the same formulation, the two vaccines can interfere.
frequently occurs with live attenuated vaccines, where one of the vaccine
components is more robust than the others and suppresses the growth and
immune response to the other
This phenomenon was first noted in the trivalent Sabin
polio vaccine, where the amount of serotype 2 virus in the vaccine had to be
reduced to stop it from interfering with the "take" of the serotype 1 and 2
viruses in the vaccine.
SIVagm is a lentivirus also a
The genome consists of two strands, a longer negative-sense
strand and a shorter and positive-sense strand of variable length.
the virion these strands are arranged such that the two ends of the long strand
meet but are not covalently bonded
The virus binds to receptors allowing viral particles to enter
This is then translocated to the nucleus, where the
partially double stranded DNA is 'repaired' by the
viral polymerase to
form a complete circular dsDNA genome (called covalently-closed-circular DNA or
The genome then undergoes transcription
by the host cell RNA polymerase and the pregenomicRNA (pgRNA) is sent out of
A polymerase is an enzyme that
synthesizes long chains of
(DNA polymerase and RNA polymerase are used to assemble
DNA and RNA molecules, respectively, by copying a DNA template strand using
base-pairing interactions or RNA by half ladder replication.)
is inserted into an assembled viral capsid containing the viral polymerase.
Inside this capsid the genome is converted from RNA to pdsDNA through
activity of the polymerase as an RNA-dependent-DNA-polymerase and subsequently
as an RNAse to eliminate the pgRNA transcript.
These new virions either
leave the cell to infect others or are immediately dismantled so the new viral
genomes can enter the nucleus and magnify the infection.
that leave the cell egress through budding.
This is a deoxyribonucleic
1960s Researchers "isolate" and then inoculate with the MS-2 strain of
hepatitis B virus.
Hepatitis viruses replicates through an RNA
intermediate form by reverse transcription, and in this respect they are
similar to retroviruses.
Hepatitis B virus belongs to the Hepadnavirus
Hepadnaviruses have very small genomes of partially
double-stranded, partially single stranded circular DNA.
Was There an AIDS Contract?
What Really Caused The AIDS Epidemic?
early 1970s African rhesus monkeys are used in the manufacture
of the hepatitis B vaccine.
Human hepatitis B viruses cultured
in vivo in rhesus monkeys are
returned to humans whose infected blood serum is then pooled to develop four
different strains of experimental hepatitis B vaccine.
experimental vaccine is pilot tested in New York City and central
Hepatitis B vaccine
recipients worldwide, including gay men in
New York, and Black
Africans in Central Africa, are exposed to simian viruses including SV40,
SIVagm, and perhaps others.
A generally neglected evolutionary route of
SIVagm to HIV-1 zoonosis sequentially involves:
a) human incubation for
more than a decade of polio vaccine contaminants and
recombinants including SV40, SFR, and
b) the pooling of infected blood serum donated by
hundreds of gay American and Black African
hepatitis B vaccine recipients who
had subsequently received injections with cultured strains of hepatitis B
c) the biohazardous laboratory conditions and viral
containment problems reported by the hepatitis B vaccine investigators and their
This series of events provides the best explanation for an
early to mid-1970s "punctuated origin event" most precisely fitting
the etiological determinations of the
There is evidence demonstrating
that the schizophrenia associated
retrovirus (SZRV) is an autoimmune
disorder causing retrovirus in the Type-D family of retroviruses, e.g.,
SRV-1 (simian retrovirus type 1), SRV-2 (simian retrovirus type 2), M7 (baboon
endogenous retrovirus), SMRV-H (squirrel monkey retrovirus), HTLV (human T lymphocyte leukaemia virus) and
distantly-related to HIV (human
1972World Health Organization
Bulletin No. 47 refers to creation of an immune virus and suggests that a
useful way to study the effects would be "to put it into a vaccination program
and observe the results".
Curiously the spread of HIV infection in
Central Africa coincides precisely with
an intense smallpox vaccination
Information on the Central African countries most
infected with HIV precisely matches WHO
figures indicating the number of people vaccinated in these areas.
Documents Locked Up for 30 Years Prove This Vaccine
|HIV vaccine development
presents unprecedented challenges on multiple levels, a reality, often
overlooked, that cannot be overstated. The chief challenge is that HIV is a
human retrovirus that replicates by irreversibly inserting its genes into the
host genome. Thus, HIV infection is established permanently in a matter of days
or perhaps even hours, and it cannot be cleared by primary or anamnestic
responses that occur after exposure. In addition to integrating into the host
genome, a second unique challenge is that HIV replicates in CD4+ T cells that
are key players in protective immunity not only to HIV itself but also to many
other pathogens (cf. ref. 10). These central features distinguish the path to
an HIV vaccine from the traditional design principles that led to successful
vaccines against other infectious agents. The inability of these principles to
yield an HIV vaccine became abundantly clear in six large HIV vaccine trials,
where efficacy was not observed. Strikingly, vaccination increased the risk of
infection in two of these studies that selectively targeted T-cell immunity,
providing a stark contrast between the development of conventional and HIV
Against this backdrop, what will it take to develop the first
protective vaccine against a human retrovirus?
1983 A French team at the Pasteur Institute in Paris,
France, led by Luc Montagnier, publish a paper in Science describing a
retrovirus they call LAV (lymphadenopathy associated
virus), isolated from a patient at risk for
May 6, 1983 "The disease was first
believed to be confined to a particular epidemiologically defined segment of
Earlier hypotheses suggested there was something within
the lifestyle of male homosexuals that predisposed them to this
Theories put forth suggesting drugs such as amyl nitrite,
antigenic overload, and medications taken for the multiple infections affecting
male homosexuals." - Anthony S. Fauci, National Institutes of Health; Acquired Immune Deficiency Syndrome: Ever-Broadening Clinical
May 4, 1984 Robert Gallo, a
researcher at the National Cancer
Institute where he worked for 30 years mainly as head of the
Laboratory of Tumor Cell Biology, and collaborators publish a series of
four papers in Science demonstrating that a retrovirus they claim to
have isolated is the cause of AIDS, HTLV-III, related to the
leukaemia viruses of Gallo's
Method of continuous production of retroviruses
1987 Out of court settlement
between the National Institutes of
Health (NIH)and Pasteur Institute in Paris.
1991 Following years of controversy surrounding the out of
court settlement between the National Institutes of Health and the
Pasteur Institute, Gallo admits the virus he claimed to have discovered
in 1984 is in reality a virus sent to him from France the year before, putting
an end to a six-year effort by Gallo and his employer, the National
Institutes of Health, to claim the AIDS virus as
1993 "This paper shows how to
equate different aspects of imperfection in a prophylactic vaccine in terms of
impact upon levels of herd immunity, and hence upon the vaccine coverage
required for eradication.
that an otherwise perfect vaccine that gives protection which wanes with a
half-life of 10 years is only as good as a vaccine that works in 30% of people
giving them complete, lifelong protection." - Imperfect vaccines and herd immunity to HIV
1995 Gallo with his colleagues Paolo Lusso and Fiorenza Cocchi
publish their discovery
that chemokines, a class of naturally occurring compounds, are potent and
specific HIV inhibitors.
This discovery was heralded by Science
magazine as one of the top scientific breakthroughs of the year.
role chemokines play in controlling the progression of HIV infection influences
thinking on how AIDS works against the
1996 Gallo, Robert R. Redfield and
William A. Blattner, found the Institute of Human Virology.
2007 Gallo and his team are awarded a $15 million grant from
the Gates Foundation for
research into a preventive vaccine for HIV/AIDS.
2011 Gallo and his team received $23.4 million from a
consortium of funding sources to support the next phase of research into the
Institute of Human Virology's (IHV) promising HIV/AIDS preventive vaccine
The IHV vaccine program grants included $16.8 million from
the Gates Foundation, $2.2
million from the U.S. Army's Military HIV Research Program (MHRP), and other
research funding from a variety of sources including the National Institutes
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