Meet Bill Gates

Genetic Dark Matter:
Return of the Goddess

Every living thing is connected via webs of genetic and epigenetic relationships.

MicroRNAs function as cross-kingdom master regulators of the fellowship of the biosphere.

Produced by bacteria, fungi, plants, and animals, microRNAs are capable of surviving digestive and assimilative processes intact, entering the tissues of organisms, affecting the expression of a wide range of genes.

Immunopathogenesis of HIV infection

COVID-19: genetic network analysis

"The viral network we have detailed is a snapshot of the early stages of an epidemic,
before the evolutionary paths of COVID-19 become obscured by vast numbers of mutations.
It's like catching an incipient supernova in the act." - Peter Forster

Vehicles of intercellular communication

Viral surface glycoproteins, gp120 and gp41, as potential drug targets against HIV-1

Chimpanzees Immunized with Human Immunodeficiency Virus Envelope Glycoprotein

Simian Virus 40 (SV40):
A Cancer Causing Monkey Virus from FDA-Approved Vaccines

Scientist who spiked rabbit blood to fake HIV Vaccine Results Gets Rare Prison Sentence

Bill Gates donates $10m to Australian trial of immune-boosting vaccine

Combinatorial CRISPR-Cas9 and RNA Interference Attack
on HIV-1 DNA and RNA Can Lead to Cross-Resistance

HIV envelopolype peptides

Beneficial Effects of Cannabis on Blood Brain Barrier Function in HIV

Use of antagonists of the interaction between HIV GP120 and A4B7 integrin

Boosting of HIV-1 Neutralizing Antibody Responses by a Distally Related Retroviral Envelope Protein

Select gp120 V2 domain specific antibodies derived from HIV
and SIV infection and vaccination inhibit gp120 binding to α4ß7

Role for microRNAs as inter-species
mediators of epigenetic information

MicroRNAs are short non-coding RNA molecules involved in the posttranscriptional epigenetic regulation of gene expression.

Recent data show that microRNAs can be found in body fluids, and these microRNAs might enter cells giving rise to a hormone like way of action.

MicroRNAs released in body fluids might affect other individuals, and there are some data of potential cross-species action of microRNAs.

MicroRNAs traverse the gastrointestinal tract.

MicroRNAs wander via the food-chain.

Master regulatory microRNAs may influence gene expression.

MicroRNA genes, located in the non-protein coding "dark matter" of the genome, may facilitate interindividual and cross-species epigenetic communication via information transfer by coded gene products.

HIV-infected cells persist only for a day or two.
HTLV is thought to replicate by promoting proliferation of infected cells.
"Assume about 10 years between HIV infection and diagnosis of AIDS." - ASF

Retroviral integration and human gene therapy

HIV in gene therapy to fix ‘bubble boy’ disease

The simian foamy virus (SFV), a spumavirus, has been co-speciated with Old World primates for about 30 million years, making them the oldest known vertebrate RNA (ribonucleic acid) or retroviruses.

late 1950's SV40, a retrovirus, is identified in the injected form of the polio vaccine.

This is believed to be due to kidney cells from infected monkeys being used to amplify the vaccine virus during production.

Both the Sabin vaccine (oral, live virus) and the Salk vaccine (injectable, killed virus) are affected; the technique used to inactivate the polio virus in the Salk vaccine, by means of formaldehyde, did not reliably kill SV40.

When two or more vaccines are mixed together in the same formulation, the two vaccines can interfere.

This most frequently occurs with live attenuated vaccines, where one of the vaccine components is more robust than the others and suppresses the growth and immune response to the other components.

This phenomenon was first noted in the trivalent Sabin polio vaccine, where the amount of serotype 2 virus in the vaccine had to be reduced to stop it from interfering with the "take" of the serotype 1 and 2 viruses in the vaccine.

SIVagm is a lentivirus also a retrovirus.

The genome consists of two strands, a longer negative-sense strand and a shorter and positive-sense strand of variable length.

In the virion these strands are arranged such that the two ends of the long strand meet but are not covalently bonded together.

The virus binds to receptors allowing viral particles to enter the cytoplasm.

This is then translocated to the nucleus, where the partially double stranded DNA is 'repaired' by the viral polymerase to form a complete circular dsDNA genome (called covalently-closed-circular DNA or cccDNA).

The genome then undergoes transcription by the host cell RNA polymerase and the pregenomicRNA (pgRNA) is sent out of the nucleus.

A polymerase is an enzyme that synthesizes long chains of polymers.

(DNA polymerase and RNA polymerase are used to assemble DNA and RNA molecules, respectively, by copying a DNA template strand using base-pairing interactions or RNA by half ladder replication.)

The pgRNA is inserted into an assembled viral capsid containing the viral polymerase.

Inside this capsid the genome is converted from RNA to pdsDNA through activity of the polymerase as an RNA-dependent-DNA-polymerase and subsequently as an RNAse to eliminate the pgRNA transcript.

These new virions either leave the cell to infect others or are immediately dismantled so the new viral genomes can enter the nucleus and magnify the infection.

The virions that leave the cell egress through budding.

This is a deoxyribonucleic acid virus.

The role of exosomal transport of viral agents

Exosomes in Human Immunodeficiency Virus Type I Pathogenesis

Another HIV vaccine strategy fails in large-scale study

Antibody persistence and T-cell balance: Key factors confronting HIV vaccine development

late 1960s Researchers "isolate" and then inoculate with the MS-2 strain of hepatitis B virus.

Hepatitis viruses replicates through an RNA intermediate form by reverse transcription, and in this respect they are similar to retroviruses.

Hepatitis B virus belongs to the Hepadnavirus family.

Hepadnaviruses have very small genomes of partially double-stranded, partially single stranded circular DNA.

Was There an AIDS Contract?

What Really Caused The AIDS Epidemic?

early 1970s African rhesus monkeys are used in the manufacture of the hepatitis B vaccine.

Human hepatitis B viruses cultured in vivo in rhesus monkeys are returned to humans whose infected blood serum is then pooled to develop four different strains of experimental hepatitis B vaccine.

This experimental vaccine is pilot tested in New York City and central Africa.

Hepatitis B vaccine recipients worldwide, including gay men in New York, and Black Africans in Central Africa, are exposed to simian viruses including SV40, SIVagm, and perhaps others.

A generally neglected evolutionary route of SIVagm to HIV-1 zoonosis sequentially involves:

a) human incubation for more than a decade of polio vaccine contaminants and recombinants including SV40, SFR, and possibly SIVagm;

b) the pooling of infected blood serum donated by hundreds of gay American and Black African hepatitis B vaccine recipients who had subsequently received injections with cultured strains of hepatitis B virus;

c) the biohazardous laboratory conditions and viral containment problems reported by the hepatitis B vaccine investigators and their affiliates;

This series of events provides the best explanation for an early to mid-1970s "punctuated origin event" most precisely fitting the etiological determinations of the HIV-1/AIDS pandemic.

There is evidence demonstrating that the schizophrenia associated retrovirus (SZRV) is an autoimmune disorder causing retrovirus in the Type-D family of retroviruses, e.g., SRV-1 (simian retrovirus type 1), SRV-2 (simian retrovirus type 2), M7 (baboon endogenous retrovirus), SMRV-H (squirrel monkey retrovirus), HTLV (human T lymphocyte leukaemia virus) and distantly-related to HIV (human immunodeficiency virus).

1972World Health Organization Bulletin No. 47 refers to creation of an immune virus and suggests that a useful way to study the effects would be "to put it into a vaccination program and observe the results".

Curiously the spread of HIV infection in Central Africa coincides precisely with an intense smallpox vaccination campaign.

Information on the Central African countries most infected with HIV precisely matches WHO figures indicating the number of people vaccinated in these areas.

Government Documents Locked Up for 30 Years Prove This Vaccine Unsafe

mRNA Platform

Imperfect vaccines and herd immunity to HIV

Development and Evaluation of a Vaccine for HIV Infection

Another HIV Vaccine Fails a Trial, Disappointing Researchers

Antibody persistence and T-cell balance:
Two key factors confronting HIV vaccine development

HIV vaccine development presents unprecedented challenges on multiple levels, a reality, often overlooked, that cannot be overstated. The chief challenge is that HIV is a human retrovirus that replicates by irreversibly inserting its genes into the host genome. Thus, HIV infection is established permanently in a matter of days or perhaps even hours, and it cannot be cleared by primary or anamnestic responses that occur after exposure. In addition to integrating into the host genome, a second unique challenge is that HIV replicates in CD4+ T cells that are key players in protective immunity not only to HIV itself but also to many other pathogens (cf. ref. 10). These central features distinguish the path to an HIV vaccine from the traditional design principles that led to successful vaccines against other infectious agents. The inability of these principles to yield an HIV vaccine became abundantly clear in six large HIV vaccine trials, where efficacy was not observed. Strikingly, vaccination increased the risk of infection in two of these studies that selectively targeted T-cell immunity, providing a stark contrast between the development of conventional and HIV vaccines.

Against this backdrop, what will it take to develop the first protective vaccine against a human retrovirus?

Cannabis in HIV-associated sensory neuropathy: a randomized placebo-controlled trial

Cannabinoid inhibits HIV-1 adhesion of monocyte cells to extracellular matrix proteins

Pre-existing and de novo humoral immunity to SARS-CoV-2 in humans

SARS-CoV-2 T-cell epitopes define heterologous and COVID-19-induced T-cell recognition

SARS-CoV-2-specific T cell immunity in cases of COVID-19 and SARS

1983 A French team at the Pasteur Institute in Paris, France, led by Luc Montagnier, publish a paper in Science describing a retrovirus they call LAV (lymphadenopathy associated virus), isolated from a patient at risk for AIDS.

May 6, 1983 "The disease was first believed to be confined to a particular epidemiologically defined segment of the population.

Earlier hypotheses suggested there was something within the lifestyle of male homosexuals that predisposed them to this syndrome.

Theories put forth suggesting drugs such as amyl nitrite, antigenic overload, and medications taken for the multiple infections affecting male homosexuals." - Anthony S. Fauci, National Institutes of Health; Acquired Immune Deficiency Syndrome: Ever-Broadening Clinical Spectrum

May 4, 1984 Robert Gallo, a researcher at the National Cancer Institute where he worked for 30 years mainly as head of the Laboratory of Tumor Cell Biology, and collaborators publish a series of four papers in Science demonstrating that a retrovirus they claim to have isolated is the cause of AIDS, HTLV-III, related to the leukaemia viruses of Gallo's earlier work.

Method of continuous production of retroviruses (HTLV-III)

1987 Out of court settlement between the National Institutes of Health (NIH)and Pasteur Institute in Paris.

1991 Following years of controversy surrounding the out of court settlement between the National Institutes of Health and the Pasteur Institute, Gallo admits the virus he claimed to have discovered in 1984 is in reality a virus sent to him from France the year before, putting an end to a six-year effort by Gallo and his employer, the National Institutes of Health, to claim the AIDS virus as an independent discovery.

1993 "This paper shows how to equate different aspects of imperfection in a prophylactic vaccine in terms of impact upon levels of herd immunity, and hence upon the vaccine coverage required for eradication.

Such comparisons reveal that an otherwise perfect vaccine that gives protection which wanes with a half-life of 10 years is only as good as a vaccine that works in 30% of people giving them complete, lifelong protection." - Imperfect vaccines and herd immunity to HIV

1995 Gallo with his colleagues Paolo Lusso and Fiorenza Cocchi publish their discovery that chemokines, a class of naturally occurring compounds, are potent and specific HIV inhibitors.

This discovery was heralded by Science magazine as one of the top scientific breakthroughs of the year.

The role chemokines play in controlling the progression of HIV infection influences thinking on how AIDS works against the human immune system.

1996 Gallo, Robert R. Redfield and William A. Blattner, found the Institute of Human Virology.

2007 Gallo and his team are awarded a $15 million grant from the Gates Foundation for research into a preventive vaccine for HIV/AIDS.

2011 Gallo and his team received $23.4 million from a consortium of funding sources to support the next phase of research into the Institute of Human Virology's (IHV) promising HIV/AIDS preventive vaccine candidate.

The IHV vaccine program grants included $16.8 million from the Gates Foundation, $2.2 million from the U.S. Army's Military HIV Research Program (MHRP), and other research funding from a variety of sources including the National Institutes of Health.

Cannabinoids inhibit migration of microglial-like cells to the HIV protein Tat

Marijuana Smoking Does Not Accelerate Progression of Liver Disease in HIV–Hepatitis C Coinfection

chemical warfare

unique library index

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